AFLP analysis and improved phytoextraction capacity of transgenic gshI-poplar clones (Populus x canescens L.) for copper in vitro.
Identifieur interne : 003E99 ( Main/Exploration ); précédent : 003E98; suivant : 003F00AFLP analysis and improved phytoextraction capacity of transgenic gshI-poplar clones (Populus x canescens L.) for copper in vitro.
Auteurs : Gábor Gyulai [Hongrie] ; Mervyn Humphreys ; András Bittsánszky ; Kirsten Sk T ; J Zsef Kiss ; Leif Sk Tc ; Gábor Gullner ; Sue Heywood ; Zoltán Szab ; Alan Lovatt ; Lászl Radimszky ; Hywel Roderick ; Heinz Rennenberg ; Michael Abberton ; Tamás Komíves ; Lászl HeszkySource :
- Zeitschrift fur Naturforschung. C, Journal of biosciences [ 0939-5075 ]
Descripteurs français
- KwdFr :
- ADN des plantes (génétique), ADN des plantes (isolement et purification), Amorces ADN (MeSH), Amplification de gène (MeSH), Cartographie de restriction (MeSH), Cuivre (pharmacocinétique), Dépollution biologique de l'environnement (MeSH), Feuilles de plante (MeSH), Polymorphisme génétique (MeSH), Populus (métabolisme), Réaction de polymérisation en chaîne (MeSH), Séquence nucléotidique (MeSH), Végétaux génétiquement modifiés (métabolisme).
- MESH :
- génétique : ADN des plantes.
- isolement et purification : ADN des plantes.
- métabolisme : Populus, Végétaux génétiquement modifiés.
- pharmacocinétique : Cuivre.
- Amorces ADN, Amplification de gène, Cartographie de restriction, Dépollution biologique de l'environnement, Feuilles de plante, Polymorphisme génétique, Réaction de polymérisation en chaîne, Séquence nucléotidique.
English descriptors
- KwdEn :
- Base Sequence (MeSH), Biodegradation, Environmental (MeSH), Copper (pharmacokinetics), DNA Primers (MeSH), DNA, Plant (genetics), DNA, Plant (isolation & purification), Gene Amplification (MeSH), Plant Leaves (MeSH), Plants, Genetically Modified (metabolism), Polymerase Chain Reaction (MeSH), Polymorphism, Genetic (MeSH), Populus (metabolism), Restriction Mapping (MeSH).
- MESH :
- chemical , genetics : DNA, Plant.
- chemical , isolation & purification : DNA, Plant.
- chemical , pharmacokinetics : Copper.
- metabolism : Plants, Genetically Modified, Populus.
- Base Sequence, Biodegradation, Environmental, DNA Primers, Gene Amplification, Plant Leaves, Polymerase Chain Reaction, Polymorphism, Genetic, Restriction Mapping.
Abstract
Clone stability and in vitro phytoextraction capacity of vegetative clones of P. x canescens (2n = 4x = 38) including two transgenic clones (ggs11 and lgl6) were studied as in vitro leaf disc cultures. Presence of the gshI-transgene in the transformed clones was detected in PCR reactions using gshI-specific primers. Clone stability was determined by fAFLP (fluorescent amplified DNA fragment length polymorphism) analysis. In total, 682 AFLP fragments were identified generated by twelve selective primer pairs after EcoRI-MseI digestion. Four fragments generated by EcoAGT-MseCCC were different (99.4% genetic similarity) which proves an unexpectedly low bud mutation frequency in P. x canescens. For the study of phytoextraction capacity leaf discs (8 mm) were exposed to a concentration series of ZnSO4 (10(-1) to 10(-5) M) incubated for 21 days on aseptic tissue culture media WPM containing 1 microM Cu. Zn2+ caused phytotoxicity only at high concentrations (10(-1) to 10(-2) M). The transgenic poplar cyt-ECS (ggs11) clone, as stimulated by the presence of Zn, showed elevated heavy metal (Cu) uptake as compared to the non-transformed clone. These results suggest that gshI-transgenic poplars may be suitable for phytoremediation of soils contaminated with zinc and copper.
DOI: 10.1515/znc-2005-3-415
PubMed: 15948599
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Gödöllö</wicri:regionArea><wicri:noRegion>Gödöllö</wicri:noRegion></affiliation></author><author><name sortKey="Humphreys, Mervyn" sort="Humphreys, Mervyn" uniqKey="Humphreys M" first="Mervyn" last="Humphreys">Mervyn Humphreys</name></author><author><name sortKey="Bittsanszky, Andras" sort="Bittsanszky, Andras" uniqKey="Bittsanszky A" first="András" last="Bittsánszky">András Bittsánszky</name></author><author><name sortKey="Sk T, Kirsten" sort="Sk T, Kirsten" uniqKey="Sk T K" first="Kirsten" last="Sk T">Kirsten Sk T</name></author><author><name sortKey="Kiss, J Zsef" sort="Kiss, J Zsef" uniqKey="Kiss J" first="J Zsef" last="Kiss">J Zsef Kiss</name></author><author><name sortKey="Sk Tc, Leif" sort="Sk Tc, Leif" uniqKey="Sk Tc L" first="Leif" last="Sk Tc">Leif Sk Tc</name></author><author><name sortKey="Gullner, Gabor" sort="Gullner, Gabor" uniqKey="Gullner G" first="Gábor" last="Gullner">Gábor Gullner</name></author><author><name sortKey="Heywood, Sue" sort="Heywood, Sue" uniqKey="Heywood S" first="Sue" last="Heywood">Sue Heywood</name></author><author><name sortKey="Szab, Zoltan" sort="Szab, Zoltan" uniqKey="Szab Z" first="Zoltán" last="Szab">Zoltán Szab</name></author><author><name sortKey="Lovatt, Alan" sort="Lovatt, Alan" uniqKey="Lovatt A" first="Alan" last="Lovatt">Alan Lovatt</name></author><author><name sortKey="Radimszky, Laszl" sort="Radimszky, Laszl" uniqKey="Radimszky L" first="Lászl" last="Radimszky">Lászl Radimszky</name></author><author><name sortKey="Roderick, Hywel" sort="Roderick, Hywel" uniqKey="Roderick H" first="Hywel" last="Roderick">Hywel Roderick</name></author><author><name sortKey="Rennenberg, Heinz" sort="Rennenberg, Heinz" uniqKey="Rennenberg H" first="Heinz" last="Rennenberg">Heinz Rennenberg</name></author><author><name sortKey="Abberton, Michael" sort="Abberton, Michael" uniqKey="Abberton M" first="Michael" last="Abberton">Michael Abberton</name></author><author><name sortKey="Komives, Tamas" sort="Komives, Tamas" uniqKey="Komives T" first="Tamás" last="Komíves">Tamás Komíves</name></author><author><name sortKey="Heszky, Laszl" sort="Heszky, Laszl" uniqKey="Heszky L" first="Lászl" last="Heszky">Lászl Heszky</name></author></analytic><series><title level="j">Zeitschrift fur Naturforschung. C, Journal of biosciences</title><idno type="ISSN">0939-5075</idno></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Sequence (MeSH)</term><term>Biodegradation, Environmental (MeSH)</term><term>Copper (pharmacokinetics)</term><term>DNA Primers (MeSH)</term><term>DNA, Plant (genetics)</term><term>DNA, Plant (isolation & purification)</term><term>Gene Amplification (MeSH)</term><term>Plant Leaves (MeSH)</term><term>Plants, Genetically Modified (metabolism)</term><term>Polymerase Chain Reaction (MeSH)</term><term>Polymorphism, Genetic (MeSH)</term><term>Populus (metabolism)</term><term>Restriction Mapping (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN des plantes (génétique)</term><term>ADN des plantes (isolement et purification)</term><term>Amorces ADN (MeSH)</term><term>Amplification de gène (MeSH)</term><term>Cartographie de restriction (MeSH)</term><term>Cuivre (pharmacocinétique)</term><term>Dépollution biologique de l'environnement (MeSH)</term><term>Feuilles de plante (MeSH)</term><term>Polymorphisme génétique (MeSH)</term><term>Populus (métabolisme)</term><term>Réaction de polymérisation en chaîne (MeSH)</term><term>Séquence nucléotidique (MeSH)</term><term>Végétaux génétiquement modifiés (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Plant</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>DNA, Plant</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacokinetics" xml:lang="en"><term>Copper</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN des plantes</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>ADN des plantes</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Plants, Genetically Modified</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Populus</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="pharmacocinétique" xml:lang="fr"><term>Cuivre</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Biodegradation, Environmental</term><term>DNA Primers</term><term>Gene Amplification</term><term>Plant Leaves</term><term>Polymerase Chain Reaction</term><term>Polymorphism, Genetic</term><term>Restriction Mapping</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Amorces ADN</term><term>Amplification de gène</term><term>Cartographie de restriction</term><term>Dépollution biologique de l'environnement</term><term>Feuilles de plante</term><term>Polymorphisme génétique</term><term>Réaction de polymérisation en chaîne</term><term>Séquence nucléotidique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Clone stability and in vitro phytoextraction capacity of vegetative clones of P. x canescens (2n = 4x = 38) including two transgenic clones (ggs11 and lgl6) were studied as in vitro leaf disc cultures. Presence of the gshI-transgene in the transformed clones was detected in PCR reactions using gshI-specific primers. Clone stability was determined by fAFLP (fluorescent amplified DNA fragment length polymorphism) analysis. In total, 682 AFLP fragments were identified generated by twelve selective primer pairs after EcoRI-MseI digestion. Four fragments generated by EcoAGT-MseCCC were different (99.4% genetic similarity) which proves an unexpectedly low bud mutation frequency in P. x canescens. For the study of phytoextraction capacity leaf discs (8 mm) were exposed to a concentration series of ZnSO4 (10(-1) to 10(-5) M) incubated for 21 days on aseptic tissue culture media WPM containing 1 microM Cu. Zn2+ caused phytotoxicity only at high concentrations (10(-1) to 10(-2) M). The transgenic poplar cyt-ECS (ggs11) clone, as stimulated by the presence of Zn, showed elevated heavy metal (Cu) uptake as compared to the non-transformed clone. These results suggest that gshI-transgenic poplars may be suitable for phytoremediation of soils contaminated with zinc and copper.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15948599</PMID><DateCompleted><Year>2005</Year><Month>06</Month><Day>30</Day></DateCompleted><DateRevised><Year>2019</Year><Month>10</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0939-5075</ISSN><JournalIssue CitedMedium="Print"><Volume>60</Volume><Issue>3-4</Issue><PubDate><MedlineDate>2005 Mar-Apr</MedlineDate></PubDate></JournalIssue><Title>Zeitschrift fur Naturforschung. C, Journal of biosciences</Title><ISOAbbreviation>Z Naturforsch C J Biosci</ISOAbbreviation></Journal><ArticleTitle>AFLP analysis and improved phytoextraction capacity of transgenic gshI-poplar clones (Populus x canescens L.) for copper in vitro.</ArticleTitle><Pagination><MedlinePgn>300-6</MedlinePgn></Pagination><Abstract><AbstractText>Clone stability and in vitro phytoextraction capacity of vegetative clones of P. x canescens (2n = 4x = 38) including two transgenic clones (ggs11 and lgl6) were studied as in vitro leaf disc cultures. Presence of the gshI-transgene in the transformed clones was detected in PCR reactions using gshI-specific primers. Clone stability was determined by fAFLP (fluorescent amplified DNA fragment length polymorphism) analysis. In total, 682 AFLP fragments were identified generated by twelve selective primer pairs after EcoRI-MseI digestion. Four fragments generated by EcoAGT-MseCCC were different (99.4% genetic similarity) which proves an unexpectedly low bud mutation frequency in P. x canescens. For the study of phytoextraction capacity leaf discs (8 mm) were exposed to a concentration series of ZnSO4 (10(-1) to 10(-5) M) incubated for 21 days on aseptic tissue culture media WPM containing 1 microM Cu. Zn2+ caused phytotoxicity only at high concentrations (10(-1) to 10(-2) M). The transgenic poplar cyt-ECS (ggs11) clone, as stimulated by the presence of Zn, showed elevated heavy metal (Cu) uptake as compared to the non-transformed clone. These results suggest that gshI-transgenic poplars may be suitable for phytoremediation of soils contaminated with zinc and copper.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Gyulai</LastName><ForeName>Gábor</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>St. Stephanus University, Department of Genetics and PB, Gödöllö, Hungary. gyulai.gabor@mkk.szie.hu</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Humphreys</LastName><ForeName>Mervyn</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Bittsánszky</LastName><ForeName>András</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Skøt</LastName><ForeName>Kirsten</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Kiss</LastName><ForeName>József</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Skøtc</LastName><ForeName>Leif</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Gullner</LastName><ForeName>Gábor</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Heywood</LastName><ForeName>Sue</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Szabó</LastName><ForeName>Zoltán</ForeName><Initials>Z</Initials></Author><Author ValidYN="Y"><LastName>Lovatt</LastName><ForeName>Alan</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Radimszky</LastName><ForeName>László</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Roderick</LastName><ForeName>Hywel</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Rennenberg</LastName><ForeName>Heinz</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Abberton</LastName><ForeName>Michael</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Komíves</LastName><ForeName>Tamás</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Heszky</LastName><ForeName>László</ForeName><Initials>L</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Z Naturforsch C J Biosci</MedlineTA><NlmUniqueID>8912155</NlmUniqueID><ISSNLinking>0341-0382</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017931">DNA Primers</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018744">DNA, Plant</NameOfSubstance></Chemical><Chemical><RegistryNumber>789U1901C5</RegistryNumber><NameOfSubstance UI="D003300">Copper</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001673" MajorTopicYN="Y">Biodegradation, Environmental</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003300" MajorTopicYN="N">Copper</DescriptorName><QualifierName UI="Q000493" MajorTopicYN="Y">pharmacokinetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017931" MajorTopicYN="N">DNA Primers</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018744" MajorTopicYN="N">DNA, Plant</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005784" MajorTopicYN="N">Gene Amplification</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="Y">Plants, Genetically Modified</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016133" MajorTopicYN="N">Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011110" MajorTopicYN="Y">Polymorphism, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015183" MajorTopicYN="N">Restriction Mapping</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2005</Year><Month>6</Month><Day>14</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2005</Year><Month>7</Month><Day>1</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2005</Year><Month>6</Month><Day>14</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">15948599</ArticleId><ArticleId IdType="doi">10.1515/znc-2005-3-415</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Hongrie</li></country></list><tree><noCountry><name sortKey="Abberton, Michael" sort="Abberton, Michael" uniqKey="Abberton M" first="Michael" last="Abberton">Michael Abberton</name><name sortKey="Bittsanszky, Andras" sort="Bittsanszky, Andras" uniqKey="Bittsanszky A" first="András" last="Bittsánszky">András Bittsánszky</name><name sortKey="Gullner, Gabor" sort="Gullner, Gabor" uniqKey="Gullner G" first="Gábor" last="Gullner">Gábor Gullner</name><name sortKey="Heszky, Laszl" sort="Heszky, Laszl" uniqKey="Heszky L" first="Lászl" last="Heszky">Lászl Heszky</name><name sortKey="Heywood, Sue" sort="Heywood, Sue" uniqKey="Heywood S" first="Sue" last="Heywood">Sue Heywood</name><name sortKey="Humphreys, Mervyn" sort="Humphreys, Mervyn" uniqKey="Humphreys M" first="Mervyn" last="Humphreys">Mervyn Humphreys</name><name sortKey="Kiss, J Zsef" sort="Kiss, J Zsef" uniqKey="Kiss J" first="J Zsef" last="Kiss">J Zsef Kiss</name><name sortKey="Komives, Tamas" sort="Komives, Tamas" uniqKey="Komives T" first="Tamás" last="Komíves">Tamás Komíves</name><name sortKey="Lovatt, Alan" sort="Lovatt, Alan" uniqKey="Lovatt A" first="Alan" last="Lovatt">Alan Lovatt</name><name sortKey="Radimszky, Laszl" sort="Radimszky, Laszl" uniqKey="Radimszky L" first="Lászl" last="Radimszky">Lászl Radimszky</name><name sortKey="Rennenberg, Heinz" sort="Rennenberg, Heinz" uniqKey="Rennenberg H" first="Heinz" last="Rennenberg">Heinz Rennenberg</name><name sortKey="Roderick, Hywel" sort="Roderick, Hywel" uniqKey="Roderick H" first="Hywel" last="Roderick">Hywel Roderick</name><name sortKey="Sk T, Kirsten" sort="Sk T, Kirsten" uniqKey="Sk T K" first="Kirsten" last="Sk T">Kirsten Sk T</name><name sortKey="Sk Tc, Leif" sort="Sk Tc, Leif" uniqKey="Sk Tc L" first="Leif" last="Sk Tc">Leif Sk Tc</name><name sortKey="Szab, Zoltan" sort="Szab, Zoltan" uniqKey="Szab Z" first="Zoltán" last="Szab">Zoltán Szab</name></noCountry><country name="Hongrie"><noRegion><name sortKey="Gyulai, Gabor" sort="Gyulai, Gabor" uniqKey="Gyulai G" first="Gábor" last="Gyulai">Gábor Gyulai</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PoplarV1
| This area was generated with Dilib version V0.6.37. |  |